In RT-FDC, fluorescence data are stored alongside the regular image and scalar features. The fluorescence data consist of the trace data (fluorescence signal over time) and several scalar features (maximum, peak position, peak width, etc.) for each fluorescence channel. The trace data are stored as raw and median-filtered traces, where median-filtered means that the raw data is filtered with a rolling median filter.
In : import dclab In : ds = dclab.new_dataset("data/example_traces.rtdc") # list the available traces in the dataset In : sorted(ds["trace"].keys()) Out: ['fl1_median', 'fl1_raw', 'fl2_median', 'fl2_raw', 'fl3_median', 'fl3_raw'] # show fluorescence meta data In : ds.config["fluorescence"]